Analisis Senyawa Bioaktif Daun Tempuyung (Sonchus arvensis L.) sebagai Kandidat Obat Herbal Antibatu Ginjal

Ardhana Yulisma; Ilda Rosa Yuliana

Authors

Ardhana Yulisma Program Studi Farmasi, Universitas U’budiyah Indonesia Author
Ilda Rosa Yuliana Program Studi Farmasi, Universitas U’budiyah Indonesia Author

Keywords:

Tempuyung (Sonchus arvensis L.), phytochemical, kidney stones, herbal medicine, flavonoid

Abstract

Nephrolithiasis, otherwise known as kidney stones, is a serious condition that is becoming increasingly prevalent, particularly in developing countries such as Indonesia. This increase in cases can be attributed to a combination of unhealthy lifestyles and genetic factors. Despite the efficacy of conservative therapy for nephrolithiasis, its side effects have led to the need for alternative treatments based on natural ingredients. The objective of this study was to analyze the active compounds present in tempuyung leaves and to evaluate their potential as a herbal remedy for kidney stones. The experimental laboratory techniques employed included extraction, phytochemical testing, quantitative analysis, and data analysis. The results of the study on the tempuyung leaf extract demonstrated the presence of bioactive compounds, including flavonoids, alkaloids, saponins, tannins, and triterpenoids, which have the potential to serve as anti-kidney stone agents. Subsequent HPLC/LC-MS analysis of the flavonoid compound group revealed that certain compounds, including quercetin, luteolin, apigenin, rutin, and kaempferol, possess anti-inflammatory, nephroprotective, antioxidant, and kidney stone dissolution properties. Consequently, tempuyung can be posited as a potential candidate for consideration in the development of phytopharmaceuticals for the treatment of kidney stones.

References

[1] Suliska N, Achadiyani I, and Imelda, “Nephroprotective effect of ethanol extract of Sonchus arvensis L. leaves in gentamicin-piroxicam induced rat renal failure,” J Res Pharm. Vol. 25, No. 1, pp. 1–8, 2021.

[2] Parisa N, Wahyuni DK, Sumantri I, et al, “Evaluation of the anti-gout effect of Sonchus Arvensis on monosodium urate crystal-induced gout arthritis via anti-inflammatory action.,” J. Res Pharm Sci. vol.16, no. 8:e0254804, 2021.

[3] Shastri, Shani et al, “Kidney Stone Pathophysiology, Evaluation and Management: Core Curriculum,” American Journal of Kidney Diseases, Vol. 82, Issue 5, pp. 617 – 634, 2023.

[4] Chhiber N., Sharma M., Kaur T., Singla S. Mineralization in health and mechanism of kidney stone formation. International Journal of Pharmaceutical Science Invention. 2014;3:25–31. [Google Scholar][Ref list]

[5] Cunningham P., Noble H., Al-Modhefer A.-K., Walsh I. Kidney stones: pathophysiology, diagnosis and management. British Journal of Nursing. 2016;25(20):1112–1116. doi: 10.12968/bjon.2016.25.20.1112. [DOI] [PubMed] [Google Scholar]

[6] Hidayat R, Sari R, Rafi M, et al. Tempuyung Leaves (Sonchus arvensis): Traditional Use, Phytochemical Composition, and Biological Activities. Open Access Maced J Med Sci. 2020;8(10):1–6.

[7] Suliska N, Achadiyani I, Imelda. Protective effect of ethanolic extract of Sonchus arvensis L. leaves in gentamicin-piroxicam induced rat renal failure. J Res Pharm Sci. 2021;16(8):e0254804.

[8] Asif A, Saeed M. Sonchus arvensis (L.) ssp arvensis (D.C.). Acta Pharm Sci. 2020;58(3):306–312.

[9] Wahyuni DK, Sumantri I, Parisa N, et al. Evaluation of the anti-gout effect of Sonchus Arvensis on monosodium urate crystal-induced gout arthritis via anti-inflammatory action. J Res Pharm Sci. 2021;16(8):e0254804.

[10] Khan RA, Khan MR, Lee JH, et al. Evaluation of flavonoids and diverse antioxidant activities of Sonchus arvensis L. Pharmacogn Mag. 2012;8(31):1–6.

[11] Rafi M, Sari R, Hidayat R, et al. Phytochemical profiling using FTIR- and LC-HRMS-based metabolomics, mineral content, and free radical scavenging activity of Sonchus arvensis extracts from different growth locations. Veg. Sci. 2025;12(2):1–12.

[12] Dhianawaty D, Dachriyanus D, Soemardji AA. The use of Sonchus arvensis leaves decoction as diuretic and anti-urolithiasis agent in Wistar rats. Med J Indones. 2008;17(4):221–225. doi:10.13181/mji.v17i4.329

[13] Widyawati T, Ruslin, Wahyuningsih MSH, et al. Preventive effect of Sonchus arvensis L. leaves extract on calcium oxalate crystal formation and renal damage in rat model. Pharmacogn Mag. 2017;13(Suppl 2):S227–S232. doi:10.4103/pm.pm_121_16

[14] Hidayati N, Handayani D, Sukandar EY. Nephroprotective activity of Sonchus arvensis L. extract on ethylene glycol-induced urolithiasis in rats. J Appl Pharm Sci. 2020;10(5):75–81. doi:10.7324/JAPS.2020.105010

[15] Wahyuni DK, Sumantri I, Parisa N, et al. Antioxidant, antimicrobial, and antiplasmodial activities of Sonchus arvensis L. leaf ethyl acetate fractions. Pharmacogn J. 2022;14(6):993–1001.

[16] Khuluk RH, Anggraeni Y, Saputri FC, et al. An HPLC-DAD method to quantify flavonoids in Sonchus arvensis and correlation with antioxidant activity. Separations. 2021;8(2):12. doi:10.3390/separations8020012

[17] Imelda I, Azaria C, Lucretia T. Protective effect of ethanolic extract Tempuyung leaf (Sonchus arvensis L.) in gentamicin-induced acute tubular necrosis on Wistar rats. Indonesian J Pharm / J Med Health (repositori/universitas). 2017–2018; (see article/pdf for details).

[18] Azimi A, Eidi A, Mortazavi P, Rohani AH. Protective effect of apigenin on ethylene glycol-induced urolithiasis via attenuating oxidative stress and inflammatory parameters in Wistar rats. Life Sci. 2021;279:119641. doi:10.1016/j.lfs.2021.119641

[19] Ye Z, Yang S, Chen L, et al. Luteolin alleviates calcium oxalate crystal-induced kidney injury by regulating oxidative stress and apoptosis pathways. Phytomedicine. 2025;136:156302. doi:10.1016/j.phymed.2025.156302

[20] Leslie SW, Bashir K. Hypocitraturia and Renal Calculi. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2024. Available from: https://www.ncbi.nlm.nih.gov/books/NBK537205/

[21] Harborne JB. Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis. 3rd ed. London: Chapman and Hall; 1998.

[22] W.P., Winarto. Tempuyung:Tanaman Penghancur Batu Ginjal.Jakarta:Agromedia Pustaka. Hal 1-3, 2004.

[23] Sunanto, Hardi.100 Resep sembuhkan Hipertensi, Obesitas, dan Asam Urat.Jakarta:PT Elex Media Komputindo. Hal 61, 2001.

[24] Sa'adah, Sumiati. Mengenal Tanaman yang Berkhasiat Obat.Jakarta:Azka Press . Hal 15, 2007

[25] H. Inventaris Tanaman Obat Indonesia. Jakarta: Puslitbang Farmasi Depkes RI; 2009.

[26] Syarifuddin H, Yusriadi, Irwan. Isolasi dan Identifikasi Senyawa Flavonoid Ekstrak n-Butanol Daun Tempuyung (Sonchus arvensis L.). Jurnal Fitofarmaka Indonesia. 2021;8(2):143-151. doi:10.33096/jffi.v8i2.785

[27] Prasetyo BF, Sari R, Rafi M. Total phenolic, flavonoid content and antioxidant activity of Sonchus arvensis L. leaf extract. Pharmacogn J. 2020;12(5):1086–1092. doi:10.5530/pj.2020.12.152

[28] Hossain MA, Shah MD, Gnanaraj C, Iqbal M. In vitro total phenolics, flavonoids contents and antioxidant activity of essential oil, various organic extracts from leaves of tropical medicinal plant Tetrastigma from Sonchus arvensis L. Asian Pac J Trop Med. 2011;4(9):717–721. doi:10.1016/S1995-7645(11)60180-8

[29] Raflizar, Mun’im A, Andrajati R, et al. Total phenolic, flavonoid contents and antioxidant activity of Sonchus arvensis L. leaf extracts. Pharmacogn J. 2018;10(1):123–128. doi:10.5530/pj.2018.1.21