Pembuatan Glukosa dari Fiber Cake Sawit dengan Metode Hidrolisis Enzim Selulase

Tithania Maharani Putri Wijaya; Martha Aznury; Cindi Ramayanti

Authors

Tithania Maharani Putri Wijaya Politeknik Negeri Sriwijaya Author
Martha Aznury Politeknik Negeri Sriwijaya Author
Cindi Ramayanti Politeknik Negeri Sriwijaya Author

Keywords:

Fiber Cake Sawit, Lignin, Enzim Selulase, Delignifikasi, Hidrolisis Enzimatis

Abstract

Palm fiber cake is waste from the palm oil processing process after going through the oil extraction process from oil palm fruit. Fiber cake contains lignocellulose in the form of cellulose, hemicellulose, and lignin. The high cellulose content in fiber cake will be used to produce monosaccharides to produce glucose. Lignin contained in palm fiber cake needs to be removed to extract cellulose because lignin functions as a protector that will prevent enzymes from breaking down cellulose into glucose. The delignification process is carried out to remove lignin levels, using H₂O₂ and MnSO₄.H₂O as catalysts to accelerate the reaction process. To convert cellulose into glucose, an enzymatic hydrolysis process is carried out using the cellulase enzyme. This study aims to determine the glucose levels produced through the hydrolysis process by varying the cellulase enzyme by 5%, 10%, and 15% of the weight of delignified palm fiber cake, with sampling times at 6, 12, and 18 hours. Based on the analysis results, the highest glucose results were obtained in sample C3 (enzyme concentration 15%/18 hours), which was 9% in the Luff Schoorl analysis and a brix value of 12°Bx with a pH value of 4.

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