Sintesis dan Karakterisasi Nanopartikel Perak Berbasis AgNO3 dan Ekstrak Daun Lamtoro (Leucaena Leucocephala) Untuk Aktivitas Antibakteri

Ayu Rosyida Zam Zam; Nashrul Amin; Sri Redjeki

Authors

Ayu Rosyida Zam Zam Universitas Pembangunan Nasional “Veteran” Jawa Timur Author
Nashrul Amin Universitas Pembangunan Nasional “Veteran” Jawa Timur Author
Sri Redjeki Universitas Pembangunan Nasional “Veteran” Jawa Timur Author https://orcid.org/0009-0006-2212-4946

Keywords:

silver nanoparticle, leucaena leucocephala, antibacterial activity, bioreduction

Abstract

This study aims to synthesize and characterize silver nanoparticles (AgNPs) based on silver nitrate (AgNO₃) using Leucaena leucocephala leaf extract as a natural bioreducing agent, and to determine the effects of AgNO₃ concentration and stirring time on their morphology, stability, and antibacterial activity. The synthesis was carried out using AgNO₃ concentrations of 1.5, 2.5, 3.5, 4.5, and 5.5 mM, with stirring times of 2, 3, and 4 hours at room temperature. UV-Vis analysis showed that a concentration of 5.5 mM and stirring time of 3 hours produced a maximum absorption peak at approximately 430 nm, indicating the successful formation of stable silver nanoparticles. SEM analysis revealed spherical-shaped AgNPs with particle sizes ranging from 50 to 100 nm. The antibacterial activity test against Staphylococcus aureus ATCC 25923 showed an inhibition zone of 11.67 mm at 100% concentration, categorized as strong antibacterial activity. Therefore, the combination of 5.5 mM AgNO₃ concentration and 3 hours of stirring time was determined to be the optimum condition for producing stable silver nanoparticles with high antibacterial potential.

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